𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Molecular cloning, expression of Sox5 and its down-regulation of Dmrt1 transcription in Zebrafish

✍ Scribed by Shang Gao; Tao Zhang; Xiang Zhou; Yang Zhao; Qin Li; Yiqing Guo; Hanhua Cheng; Rongjia Zhou

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
622 KB
Volume
304B
Category
Article
ISSN
1552-5007

No coin nor oath required. For personal study only.

✦ Synopsis

The doublesex and mab-3-related transcription factor 1 (Dmrt1) is the founding member of a family of DM domain genes, involved in gonadogenesis. Here we report the cloning of zebrafish Sox5 and use real time PCR to show that its expression increases while expression of Dmrt1 decreases during embryogenesis. Characterization of the proximal promoter of zebrafish Dmrt1 revealed two positive and two negative regulatory regions and a Sox5-binding site. Co-transfection of dmrt1 (with or without the Sox-binding site), driving an EGFP reporter and Sox5 showed further that Sox5 bound the Dmrt1 promoter and inhibited Dmrt1 expression. This antagonistic partnership between Dmrt1 and Sox5 suggests a potential transcriptional regulatory mechanism for Dmrt1 in early embryogenesis.

πŸ“œ SIMILAR VOLUMES

Molecular cloning, sequencing and expres
Molecular cloning, sequencing and expression of the mRNA encoding human Cdx1 and Cdx2 homeobox. Down-regulation of Cdx1 and Cdx2 mRNA expression during colorectal carcinogenesis
✍ Gustavo V. Mallo; Hocine Rechreche; Jean-Marc Frigerio; Dominique Rocha; Alain Z πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 French βš– 456 KB πŸ‘ 2 views

Defining the molecular mechanisms involved in cancer formation and progression is still a major challenge in colorectal-cancer research. Our strategy was to characterize genes whose expression is altered during colorectal carcinogenesis. To this end, the phenotype of a colorectal tumour was previous

Expression of osteocalcin and its transc
Expression of osteocalcin and its transcriptional regulators core-binding factor alpha 1 and MSX2 in osteoid-forming tumours
✍ Sevan Hopyan; Nalan Gokgoz; Robert Stuart Bell; Irene Louise Andrulis; Benjamin πŸ“‚ Article πŸ“… 1999 πŸ› Elsevier Science 🌐 English βš– 618 KB

## Abstract Osteosarcoma, fibrous dysplasia, and myositis ossificans contain osteoid‐producing cells that are not necessarily morphologically typical osteoblasts. Nevertheless, these pathologic cells may share differentiation steps with osteoblasts at the molecular level. Osteocalcin, a bone‐specif

Down-regulation of the transcriptional m
Down-regulation of the transcriptional mediator subunit Med1 contributes to the loss of expression of metastasis-associated dapk1 in human cancers and cancer cells
✍ Padmaja Gade; Ashish K. Singh; Sanjit K. Roy; Sekhar P. Reddy; Dhananjaya V. Kal πŸ“‚ Article πŸ“… 2009 πŸ› John Wiley and Sons 🌐 French βš– 396 KB

## Abstract DAPK1, a ca^+2^/calmodulin regulated serine/threonine kinase, is a major tumor suppressor, whose expression is lost in multiple tumor types. However, the mechanisms contributing to it are unclear. We have recently shown that CCAAT/Enhancer binding protein‐β (C/EBP‐β) is required for the

Molecular cloning of zebrafish (Danio re
Molecular cloning of zebrafish (Danio rerio) MutS homolog 6(MSH6) and noncoordinate expression of MSH6 gene activity and G-T mismatch binding proteins in zebrafish larvae
✍ Yeh, Fu-lung ;Yan, Hwei-ling ;Wang, Shi-ya ;Jung, Ting-yi ;Hsu, Todd πŸ“‚ Article πŸ“… 2003 πŸ› John Wiley and Sons 🌐 English βš– 374 KB

## Abstract Eukaryotic MutS homolog 6(MSH6) is a DNA mismatch recognition protein associated with mismatch repair of simple base‐base mispairs and small insertion‐deletion loops. As replication or recombination errors generated during embryonic development of living organisms should be efficiently