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Molecular cloning, coding nucleotides and the deduced amino acid sequence of P-450BM-1 from Bacillus megaterium

โœ Scribed by Jian-Sen He; Richard T. Ruettinger; Hong-Ming Liu; Armand J. Fulco


Book ID
119119785
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
557 KB
Volume
1009
Category
Article
ISSN
0167-4781

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A 4.3 kb EcoRI fragment carrying the gene for cytochrome P450meg, the steroid-15 beta-monooxygenase from Bacillus megaterium ATCC 13368, was cloned and completely sequenced. The gene codes for a protein of 410 amino acids and was expressed in Escherichia coli and B. subtilis. Protein extracts from t