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Molecular cloning and tissue distribution of three estrogen receptors from the cyprinid fishVaricorhinus barbatulus

โœ Scribed by Keng-Yen Fu; Chung-Yuan Chen; Chi-Tsai Lin; Whei-Meih Chang

Publisher
Springer-Verlag
Year
2007
Tongue
English
Weight
711 KB
Volume
178
Category
Article
ISSN
0174-1578

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The reverse transcriptase-polymerase chain reaction (RT-PCR), in combination with 5ะˆ and 3ะˆ rapid amplification of cDNA ends (RACE), was used to clone a G protein-coupled receptor from turkey brain mRNA. This cDNA clone has an open reading frame of 1,311 base pairs encoding a 436-residue protein wit