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Molecular cloning and expression ofThiobacillus ferrooxidanschromosomal ribulose bisphosphate carboxylase genes inEscherichia coli

✍ Scribed by Tomonobu Kusano; Kazuyuki Sugawara; Chihiro Inoue; Nobuhiro Suzuki

Publisher
Springer
Year
1991
Tongue
English
Weight
781 KB
Volume
22
Category
Article
ISSN
0343-8651

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The coding sequence for Spirulina platensis acetohydroxy acid synthase (AHAS, EC 4.1.3.18) is shown to be contained within a 4.2 Kb ClaI fragment (ilvX) that has been cloned from a recombinant lambda library. This fragment was able to complement a suitable mutant of Escherichia coli when inserted in