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Molecular Cloning and Expression of a cDNA Encoding Lon Protease from rice (Oryza sativa)

✍ Scribed by Wei Su; Changfa Lin; Jiaxue Wu; Kegui Li; Guangming He; Xiaoyin Qian; Chun Wei; Jinshui Yang


Publisher
Springer Netherlands
Year
2006
Tongue
English
Weight
391 KB
Volume
28
Category
Article
ISSN
0141-5492

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## Summary Two types of genes (__Adk__‐a, and __Adk__‐b) encoding for adenylate kinase (AK, EC 2.7.4.3.) were isolated from the cDNA library constructed from poly(A)^+^ RNA of rice (__Oryza sativa__ L.). Two cDNAs were heterogeneous at 5β€² and 3β€² ends of non‐coding sequences and had possible polyade

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The neutral protease II (NpII) from Aspergillus oryzae is a zinc-containing metalloprotease with some unique properties. To elucidate its structure, we isolated a full-length cDNA clone for NpII. Sequence analysis reveals that NpII has a prepro region consisting of 175 amino acids preceding the matu