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Molecular cloning and characterization of Cup a 4, a new allergen from Cupressus arizonica

✍ Scribed by Yago Pico de Coaña; Nuria Parody; Miguel Ángel Fuertes; Jerónimo Carnés; Daniela Roncarolo; Renato Ariano; Joaquín Sastre; Gianni Mistrello; Carlos Alonso


Book ID
116300873
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
992 KB
Volume
401
Category
Article
ISSN
0006-291X

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Isolation, cloning, and characterization
✍ Cristiano Garino; Laurian Zuidmeer; Justin Marsh; Alison Lovegrove; Maria Morati 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 229 KB

## Abstract **Scope:** 2S albumins are the major allergens involved in severe food allergy to nuts, seeds, and legumes. We aimed to isolate, clone, and express 2S albumin from hazelnut and determine its allergenicity. **Methods:** 2S albumin from hazelnut extract was purified using size exclusion c