✦ LIBER ✦
Molecular cloning and characterization of ARO7-OSM2, a single yeast gene necessary for chorismate mutase activity and growth in hypertonic medium
✍ Scribed by Ball, S. G. ;Wickner, R. B. ;Cottarel, G. ;Schaus, M. ;Tirtiaux, C.
- Publisher
- Springer
- Year
- 1986
- Tongue
- English
- Weight
- 522 KB
- Volume
- 205
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
✦ Synopsis
The chorismate mutase structural gene, AR07, which is necessary for both phenylalanine and tyrosine biosynthesis was cloned by complementation in yeast. Genetic analysis showed that AR07 was identical to a gene necessary for growth in hypertonic medium, OSM2, which mapped nearby. After restriction mapping and subcloning of the plasmid, the cloned gene was used to detect mRNA levels in several growth conditions. Enzyme activities were measured in various genotypes. At our level of detection AR07-OSM2 is a low level constitutively expressed gene.