PCR amplification ofcDNA prepared from poly(A) + RNA from aerial parts ofArabidopsis thaliana, using degenerate nucleotide primers based on conserved regions between the large and small subunits of ADP-glucose pyrophosphorylase (AGP), yielded four different cDNAs of ca. 550 nucleotides each. Based o
Molecular cloning and characterization of ADP-glucose pyrophosphorylase cDNA clones isolated from pea cotyledons
β Scribed by Diane Burgess; Andrea Penton; Pamela Dunsmuir; Hugo Dooner
- Book ID
- 110228877
- Publisher
- Springer
- Year
- 1997
- Tongue
- English
- Weight
- 343 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0167-4412
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The cDNA cloning of a small and a large subunit of ADP-glucose pyrophosphorylase (AGPase) from sugar beet is reported. The deduced amino acid sequences are highly homologous to previously identified AGPase polypeptides from other plant species. Both subunits are encoded by low copy genes. When RNA g
We isolated a rice cDNA clone which encodes an open reading frame of 382 amino acids. Its deduced amino acid sequence corresponds to an ATP/ADP translocator protein. Its homology with a maize ATP/ADP translocator was 83.9~/o in nucleotide sequence, and 90.2~o of the amino acid level. Expression of t