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Molecular cloning and amplification of the gene for aminopeptidase N of Escherichia coli

✍ Scribed by M. Bally; M. Murgier; A. Lazdunski

Book ID
109312385
Publisher
John Wiley and Sons
Year
1983
Tongue
English
Weight
257 KB
Volume
19
Category
Article
ISSN
0378-1097

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Cloning and orientation of the gene enco
Cloning and orientation of the gene encoding aminopeptidase N in Escherichia coli
✍ Bally, Marc ;Murgier, Maryse ;Lazdunski, AndrΓ©e πŸ“‚ Article πŸ“… 1984 πŸ› Springer 🌐 English βš– 450 KB

The pepN gene, that encodes aminopeptidase N in Escherichia coli, has been cloned into the multicopy plasmid pBR322. Expression of the cloned pepN gene results in overproduction of the enzyme. The restriction map of the 6.7 Kb insert was established and the gene was further localized by analysis of