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Molecular Characterization of the Glycated Plasma Membrane Calcium Pump

✍ Scribed by F.L. González Flecha; P.R. Castello; J.J. Gagliardino; J.P.F.C. Rossi


Publisher
Springer
Year
1999
Tongue
English
Weight
331 KB
Volume
171
Category
Article
ISSN
0022-2631

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Quantitation of Plasma Membrane Calcium
✍ Marı́a Mercedes Echarte; Valeria Levi; Ana Marı́a Villamil; Rolando C. Rossi; Ju 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 127 KB

P-ATPases are characterized by the formation of acid-stable phosphorylated intermediates (EP) during their reaction cycle. We have developed a microscale method to determine EP that involves the phosphorylation of the enzyme using [gamma-(32)P]ATP and precipitation with TCA; separation of the sample