Quantitation of Plasma Membrane Calcium
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Marı́a Mercedes Echarte; Valeria Levi; Ana Marı́a Villamil; Rolando C. Rossi; Ju
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Article
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2001
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Elsevier Science
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English
⚖ 127 KB
P-ATPases are characterized by the formation of acid-stable phosphorylated intermediates (EP) during their reaction cycle. We have developed a microscale method to determine EP that involves the phosphorylation of the enzyme using [gamma-(32)P]ATP and precipitation with TCA; separation of the sample