Molecular characterization of mar, a multiple aberration region on human chromosome segment 12q13-q15 implicated in various solid tumors

✍ Scribed by Dr.Wim J. M. van de Ven; Eric F. P. M. Schoenmakers; Sylke Wanschura; Bernd Kazmierczak; Patrick F. J. Kools; Jan M. W. Geurts; Sabine Bartnitzke; Herman Van Den Berghe; Jörn Bullerdiek

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 774 KB
• Volume: 12
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/gcc.2870120410

✦ Synopsis

Chromosome arm 12q breakpoints in seven cell lines derived from primary pleomorphic salivary gland adenomas were mapped by FISH analysis relative t o nine DNA probes. These probes all reside in a 2.8 Mb genomic DNA region of chromosome segment 12q 13-q I 5 and correspond t o previously published sequence-tagged sites (STS). Their relative positions were established on the basis of YAC cloning and long range physical and STS content mapping. The 12q breakpoints of five of the cell lines were found t o be mapping within three different subregions of the 445 kb DNA interval that was recently defined as the uterine leiomyoma cluster region of chromosome I2 breakpoints (ULCRI2) between STS RM33 and RM98. All seven breakpoints appeared to map within the I .7 Mb DNA region between STS RM36 and RM 103. Furthermore, the chromosome I 2 breakpoints of three primary pleomorphic salivary gland adenomas were also found t o be mapping between RM36 and RM 103. Finally, FISH analysis of two lipoma cell lines with 12q 13-q I 5 aberrations pinpointed the breakpoints of these t o relatively small and adjacent DNA segments which, as well as those of two primary lipomas, appeared t o be located also between RM36 and RM 103. We conclude from the observed clustering of the 12q breakpoints of the three distinct solid tumor types that the I .7 Mb DNA region of the long arm of chromosome I 2 between RM36 and RM I03 is a multiple aberration region which we designate MAR. Genes Chrornosom Cancer 12:296-303 (1995).