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Molecular characterization of major and minor MHC class I and II genes in B21-like haplotypes in chickens

✍ Scribed by H R Juul-Madsen; T S Dalgaard; M Afanassieff

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
338 KB
Volume
31
Category
Article
ISSN
0268-9146

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✦ Synopsis

The major histocompatibility complex (MHC) sequences of three __B__21‐like haplotypes deriving from very different origins including the Red Jungle Fowl Gallus Gallus gallus were compared with the MHC sequences of the standard __B__21 haplotype from Scandinavian White Leghorn Gallus domesticus. The present analysis reveals two cDNA sequences for B‐F and two cDNA sequences for B‐LB for every __B__21‐like haplotype, including __B__21 itself. Contrary to expectation, no sequence polymorphism in the antigen‐binding domains of the MHC genes, between the investigated haplotypes, was found. The relative level of MHC class I molecules on the surface of leukocytes measured by flow cytometry was also analysed and found to be low in Marek's Disease (MD)‐resistant B haplotypes (__B__21 and __B__21‐like) and high in MD‐susceptible B haplotypes (__B__15 and __B__19). However, in heterozygous (resistant/susceptible) animals, the relative level was almost as high as in susceptible haplotypes.

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