Molecular characterization of cell substratum attachments in human glial tumors relates to prognostic features
✍ Scribed by Nathalie Belot; Sandrine Rorive; Isabelle Doyen; Florence Lefranc; Erik Bruyneel; Robert Dedecker; Sibel Micik; Jacques Brotchi; Christine Decaestecker; Isabelle Salmon; Robert Kiss; Isabelle Camby
- Book ID
- 102846999
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 842 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0894-1491
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✦ Synopsis
Abstract
Glioma cell attachments to substratum play crucial roles in the invasion by glioma cells of normal brain tissue. These attachments are mediated through interactions between extracellular matrix (ECM) components, integrins, focal adhesion‐linked molecules, and the actin cytoskeleton. In the present study, we investigate the molecular elements involved in cell substratum attachments in human glial tumors and their potential relationships to prognostic features. We used 10 human glioma cell lines, for which we characterized glial differentiation by means of quantitative RT‐PCR for nestin, vimentin, and GFAP mRNA. We quantitatively determined the amounts of laminin, fibronectin, vitronectin, and thrombospondin secreted by these glioma cell lines in vitro, as well as the amount of each of the eight β integrin subunits and the adhesion complex‐related molecules, including talin, vinculin, profilin, zyxin, α‐actinin, paxillin, and VASP. After quantification of the levels of migration and invasion of these 10 cell lines in vitro and, through grafts into the brains of nude mice, of their biological aggressiveness in vivo, it appeared that the levels of the β5 integrin subunit and α‐actinin were directly related to biological aggressiveness. These experimental data were clinically confirmed because increasing immunohistochemical amounts of the β5 integrin subunit and α‐actinin were directly related to dismal prognoses in the case of astrocytic tumors. In addition, we show that the β4 integrin subunit are expressed significantly more in oligodendrogliomas than in astrocytic tumors. A potential role for the β8 integrin subunit in glioma cell substratum attachments is also emphasized. GLIA 36:375–390, 2001. © 2001 Wiley‐Liss, Inc.