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Molecular characterization of a prokaryotic translation factor homologous to the eukaryotic initiation factor eIF4A

✍ Scribed by Jie Lu; Hiroyuki Aoki; M.Clelia Ganoza

Book ID
117756574
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
437 KB
Volume
31
Category
Article
ISSN
1357-2725

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Cloning, expression, and crystallization
Cloning, expression, and crystallization of a hyperthermophilic protein that is homologous to the eukaryotic translation initiation factor, eIF5A
✍ Kyeong Kyu Kim; Hisao Yokota; Rosalind Kim; Sung-Hou Kim πŸ“‚ Article πŸ“… 2008 πŸ› Cold Spring Harbor Laboratory Press 🌐 English βš– 266 KB

## Abstract A gene coding for a protein homologous to a translation initiation factor of eukaryotes, eIF5A, was cloned from __Methanococcus jannaschii__, a hyperthermophile with an optimum growth temperature of 85 Β°C. The protein was overexpressed, purified and crystallized. The crystals were obtai