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Molecular characterization and chromosomal localization of mouse Purα gene

✍ Scribed by Vandhana Muralidharan; Laura Cort; Ellen Meier; Elizabeth P. Blankenhorn; Kamel Khalili

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
127 KB
Volume
77
Category
Article
ISSN
0730-2312

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✦ Synopsis

Pur␣ is a 39-kDa sequence-specific single-stranded DNA/RNA binding protein with the ability to modulate transcription of several genes containing the Pur element in their promoter region. Human and mouse Pur␣ exhibit an extraordinary degree of conservation with only two changes at amino acid residues 49 and 306. A 15-kb genomic clone encompassing the mouse Pur␣ gene was isolated by screening the mouse genomic library, using a PCR-amplified fragment from human Pur␣ cDNA. Results from sequencing analysis confirmed the isolated genomic clone to be Pur␣ and not the other members of the Pur family, including Pur␤. Characterization of the mouse Pur␣ gene by restriction analysis/Southern blotting and sequencing revealed that the Pur␣ gene contains only one intron within the 5Ј UTR and the open reading frame was intact. Using chromosomal markers, the Pur␣ gene was mapped to chromosome 18 in mouse and rat.

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