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Molecular characteristics of “Mycobacterium canettii” the smooth Mycobacterium tuberculosis bacilli

✍ Scribed by Michel Fabre; Yolande Hauck; Charles Soler; Jean-Louis Koeck; Jakko van Ingen; Dick van Soolingen; Gilles Vergnaud; Christine Pourcel


Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
505 KB
Volume
10
Category
Article
ISSN
1567-1348

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Lipoglycans were extracted from disrupted cells of Mycobacterium tuberculosis with hot phenol-water. Hydrophobic interaction chromatography on octyl-Sepharose of the crude extract separated nucleic acids and lipoglycans. The latter were retained on the column and fractionated on elution with a propa