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Molecular characterisation of the Hyp deletion and an improved assay for its detection

โœ Scribed by Campbell R. Sheen; Genay O.W. Pilarowski; Wei Wang; Jose Luis Millan

Book ID
116325067
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
367 KB
Volume
50
Category
Article
ISSN
8756-3282

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We have developed an improved assay method to measure sphingomyelinase activity and to detect this enzyme separated on polyacrylamide gels. The assay of sphingomyelinase activity involved immobilizing \(\left[\mathbf{N}\right.\)-methyl \(\left.{ }^{14} \mathrm{C}\right]\) sphingomyelin on polyvinyld