Molecular biology and infectious diseases: The institut pasteur marks its first century
✍ Scribed by Adam S. Wilkins
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 440 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0265-9247
No coin nor oath required. For personal study only.
✦ Synopsis
PROBLEMS AND PARADIGMS
however, be uncertain, since the indirect effects of a mutation on the phenotype of an organism are difficult to assess. The development of improved in vitro systems may provide sufficient information on the chromatin alteration(s) required for transcriptional activity, to make possible the design of experiments that will unequivocally test the role played by metal ions in gene activation.
The above notions have been developed based on evidence obtained with genes transcribed by RNA-polymerase 111; hence, it is also pertinent to examine genes transcribed by RNApolymerases I and 11. Interestingly, metal-binding domains of the sort seen in TFIIIA have been found in proteins which serve as cofactors for RNApolymerase 11, like SV-40 large T, Adenovirus EIA, yeast GAL4 gene product, the glucocorticoid, estrogen, progesterone and vitamin D, receptors, and cellular erbA protein.14* 22* 23
Tests of the above hypothesis may, yield new and exciting information on the role of metal ions in chromatin organization and, possibly, insights into the mechanism(s) by which genes are regulated.