Molecular aspects of chloride cell formation inOncorhynchus. II. Isolation and characterization of gill transfer RNA's during active salt secretion
✍ Scribed by Conte, F. P.
- Book ID
- 102892433
- Publisher
- John Wiley and Sons
- Year
- 1977
- Tongue
- English
- Weight
- 712 KB
- Volume
- 199
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Abstract
The transfer ribonucleic acids (tRNAs) from gill filaments of coho salmon, Oncorhynchus kisutch, have been isolated and were separated into individual species of acceptor tRNAs by reversed‐phase column chromotography. Crude tRNAs isolated from gill filaments undergoing active salt excretion were acylated with ^14^C‐amino acids and combined with tRNAs acylated with ^3^H‐amino acids, but obtained from non‐secreting filaments. The radioactively labeled tRNA mixture was cochromatographed and the results showed significant differences in the elution profiles of tRNA^asp^ and tRNA^tyr^. These elution profiles may reflect the differentiation of the chloride cell population that is found within the gill epithelium.