Molecular Aptamer Beacons for Real-Time Protein Recognition
β Scribed by Jianwei J. Li; Xiaohong Fang; Weihong Tan
- Book ID
- 115589628
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 218 KB
- Volume
- 292
- Category
- Article
- ISSN
- 0006-291X
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We have designed a new class of molecules, which we term aptamer beacons, for detecting a wide range of ligands. Similar to molecular beacons, aptamer beacons can adopt two or more conformations, one of which allows ligand binding. A fluorescence-quenching pair is used to report changes in conformat
A homogeneous fluorescence-based molecular beacon (MB) method has been developed for real-time monitoring of in vitro transcription reactions. MB probes are structured as target-specific antisense oligodeoxynucleotides containing a proximate fluorophore-quencher pair. Upon binding to its target sequ