Molecular and Cellular Interaction of the Differentiating Antitumour Agent Dimethylcrocetin with Nuclear Retinoic Acid Receptor as Studied by Near-Infrared and Visible SERS Spectroscopy
✍ Scribed by A. Beljebbar; H. Morjani; J. F. Angiboust; G. D. Sockalingum; M. Polissiou; M. Manfait
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 255 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0377-0486
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✦ Synopsis
Fourier transform (FT) Raman and surface enhanced Raman microspectroscopy (SERS) were used as a vibrational probe for investigating, in vitro and at the cellular level, the molecular interaction of dimethylcrocetin (DMCRT) with the retinoic acid nuclear receptor RAR-c. FT-SERS results obtained in vitro with silver colloids demonstrated that DMCRT interacts speciÐcally with RAR-c. Comparison between the spectra of DMCRT in HL60 cancer cells and the in vitro DMCRT-RARc complex showed practically the same shift in wavenumber of the band at 1210 cm~1 and the same intensity ratios and The similarity between the signal I 1541 /I 1165 I 1541 /I 1210 . of DMCRT in K562 cells and in the free form is explained by either an absence of nuclear receptor or an absence of any interaction between the drug and receptor. These results seem to indicate that carotenoids could possibly activate the nuclear receptor in a similar manner to retinoids.