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Molecular and biochemical studies of virus infections in two filamentous brown algae

✍ Scribed by Anne-Kathrin Klebl; Martin Bräutigam; Michael Klein; Markus Kapp; Rolf Knippers; Dieter G. Müller

Publisher
Springer
Year
1996
Tongue
English
Weight
487 KB
Volume
326-327
Category
Article
ISSN
1573-5141

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✦ Synopsis

The filamentous marine brown algae Ectocarpus siliculosus and Feldmannia simplex are infected by host specific DNA-viruses . Under Percoll isolation, Ectocarpus siliculosus-virus (EsV)-particles maintained their infective potential .

The EsV has a circular genome of dsDNA with a size of 320 kb . A restriction map has been established . The gene of a major coat protein (gpl) was detected in a genomic library and partly sequenced . Using gpl-sequences for polymerase chain reaction (PCR) amplification analysis, EsV specific sequences could be detected in various symptom-free, clonal cultures of Ectocarpus . The PCR was also used to follow the passage of the virus genome during the meiosis of hosts . A monospecific antibody against recombinant gpl was used for immunostaining and infection experiments .

The Feldmannia simplex-virus (F1exV 1) has a circular genome with a size of 220 kb and a 43% G+C content . FsV DNA contains methylated bases . 5-methylcytosine (5 mC) makes up 12% of the total cytosines .

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