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Molecular and biochemical characterization of an induced mutation conferring imidazolinone resistance in sunflower

✍ Scribed by Carlos A. Sala; Mariano Bulos; Mariel Echarte; Sherry R. Whitt; Robert Ascenzi

Book ID
105814705
Publisher
Springer
Year
2008
Tongue
English
Weight
323 KB
Volume
118
Category
Article
ISSN
0040-5752

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We isolated 607 independent nalidixic acid-resistant mutants from Bacillus subtilis. A 163 bp DNA segment from a 5' portion of the gyrA gene was amplified from the DNA of each mutant strain. After heat denaturation, the product was subjected to gel electrophoresis to detect conformational polymorphi