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Molecular analysis of the rpoD gene of Enterococcus faecalis

✍ Scribed by Dr. Jacques Frère; Xavier Gansel; Abdellah Benachour; Yanick Auffray

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
358 KB
Volume
36
Category
Article
ISSN
0233-111X

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✦ Synopsis

The complete nucleotide sequence of the rpoD gene of Enterncoccus faecalis ATCC 19433 has been determined. This gene encodes a putative 368 amino acids (aa) polypeptide of a predicted Mr of 41842 named sigma 42. Upstream of the rpoD gene and beginning from the end of the cloned chromosomal fragment is an open reading frame encoding a putative 264-aa polypeptide. It is reminiscent of the C-terminal domain of known DNA primase from Gram-positive bacteria indicating that the E. faecalis rpoD gene is included in a macromolecular synthesis (MMS) operon, as it was described for Escherichiu coli, Bacillus subtilis and Lactococcus lactis rpoD gene.

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