๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Molecular analysis of the extent of asymmetry in two asymmetric somatic hybrids of tomato

โœ Scribed by J. M. Melzer; M. A. O'Connell

Publisher
Springer
Year
1990
Tongue
English
Weight
722 KB
Volume
79
Category
Article
ISSN
0040-5752

No coin nor oath required. For personal study only.

โœฆ Synopsis

Two somatic hybrid plants generated from a single fusion event between Lycopersicon esculentum and irradiated L. pennellii protoplasts have been analyzed at the molecular level. Over 30 loci have been analyzed using isozymes and RFLPs. All loci tested on chromosomes 2-10 were heterozygous, while those loci on chromosome 12 were homozygous L. pennellii in both somatic hybrids. In one of the somatic hybrids, 2850, loci on chromosome 1 were also homozygous L. pennellii. The other somatic hybrid, 28F5, was heterozygous at all chromosome 1 loci tested, but exhibited altered stoichiometry of parental bands as compared to the sexual hybrid. Loci on chromosome 2 from both somatic hybrids have altered stoichiometry, with L. pennellii alleles being four times more abundant than expected. Both somatic hybrids contain the L. esculentum chloroplast genome, while only L. pennellii polymorphisms have been detected in the mitochondrial genome.

๐Ÿ“œ SIMILAR VOLUMES

Molecular analysis of the nuclear organe
Molecular analysis of the nuclear organellar genotype of somatic hybrid plants between tomato (Lycopersicon esculentum) andLycopersicon chilense
โœ Augusta B. Bonnema; Mary A. O'Connell ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Springer ๐ŸŒ English โš– 556 KB

Somatic hybrid plants were recovered following fusion of leaf mesophyll protoplasts isolated from tomato (Lycopersicon esculentum) cultivar UC82 with protoplasts isolated from suspension cultured cells of L. chilense, LA 1959. Iodoacetate was used to select against the growth of unfused tomato proto

Molecular analysis of single copy and re
Molecular analysis of single copy and repetitive genes on chromosome 2 in intergeneric tomato somatic hybrid plants
โœ Patrick P. Moore; Kenneth C. Sink ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Springer ๐ŸŒ English โš– 588 KB

Restriction fragment polymorphisms were used to identify and quantify the nuclear contributions from each parent to somatic hybrid plants between tomato (Lycopersicon esculentum Mill.) cv. Sub-Arctic Maxi and Solanum lycopersicoides Dun. Three single-copy clones, 2-13, 2-17, and 3-288, and a clone f

Analysis of chloroplast and mitochondria
Analysis of chloroplast and mitochondrial DNAs in asymmetric somatic hybrids between tobacco and carrot
โœ M. A. Smith; A. Pay; D. Dudits ๐Ÿ“‚ Article ๐Ÿ“… 1989 ๐Ÿ› Springer ๐ŸŒ English โš– 573 KB

Chloroplast and mitochondrial DNAs have been examined by comparison of restriction enzyme patterns in asymmetric hybrid plants, resulting from the fusion between leaf mesophyll protoplasts of Nicotiana tabacum (Solanaceae), and irradiated cell culture protoplasts of Daucus carota (Umbellifereae). Th