Molecular analysis of the cyclin-dependent kinase inhibitor genes p15INK4b/MTS21, p16INK4/MTS1, p18 and pl9 in human cancer cell lines

✍ Scribed by Akihiko Gemma; Seiichi Takenoshita; Koichi Hagiwara; Aikou Okamoto; Elisa A. Spillare; Mary G. McMemamin; S. Perwez Hussain; Kathleen Forrester; Maimoona Zariwala; Yue Xiong; Curtis C. Harris

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: French
• Weight: 660 KB
• Volume: 68
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/(sici)1097-0215(19961127)68:5<605::aid-ijc9>3.0.co;2-2

✦ Synopsis

Cyclin-dependent kinillre-4 inhibitor genes (INK4) regulate the cell cycle and are candidate tumor-suppressor genes. To determine if alterations in the coding regions of the p18 and p l 9 genes, which are novel members of the INK4 family and if they correlate with the development of human cancer, 100 human cancer cell lines were analyzed. Two other INK4 gene family members, plSINK4lMn2 and p/61w4/M"1 genes were also analyzed. Homozygous deletions of the p151NK4b/Mn2 gene were detected in 29 cancer cell lines. Thirty-five homozygcws deletions and 7 intragenic mutations of the p/6'NK41MTS' gene were also detected in these cell lines. Neither homozygous deletions nor intragmic mutations of the p18 and p l 9 genes were found except in an ovarian cancer cell line, SKOV3, harboring a single base pair deletion in exon I of p19. In p/6'NK41MTS' expression ana is, 5 cell lines with both authentic and alternative spliced