Molecular analysis of the Bacillus subtilis recF function

✍ Scribed by Alonso, Juan C. ;Stiege, A. Carola

• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 922 KB
• Volume: 228
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00260632

✦ Synopsis

recF resides between the dnaN and gyrB genes of Bacillus subtilis. The recF15 mutation results in replacement of a glutamate residue in the wild type with a lysine residue in the mutant RecF protein. We investigated the in vivo regulation of recF using a transcriptional fusion to the xylE gene and assaying mRNA production. We found that novobiocin leads to a four-fold induction in recF gene expression, but this is not observed in a gyrB mutant strain. Enhancement of expression of the recF gene in the presence of novobiocin is unrelated to the SOS response. The RecF protein, which has a predicted molecular mass of 42.2 kDa, does not seem to be involved in its own regulation.