Molecular analysis of occupational cancer: infrequent p53 and ras mutations in renal-cell cancer in workers exposed to gasoline
✍ Scribed by Stina Roth; Timo Partanen; Tuula Suitiala; Sisko Anttila; Anneli Ojajärvi; Kari Hemminki; Harri Vainio; Kirsti Husgafvel-Pursiainen
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- French
- Weight
- 103 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Occupational exposure to gasoline has been identified in several studies as a risk factor for renal-cell cancer. Cases of renal-cell cancer with and without work-related exposure to gasoline or gasoline and diesel fuel were studied for the presence of mutations in the tumour-suppressor gene p53 (n ؍ 23 exposed and 30 non-exposed cases studied) and ras oncogene (n ؍ 30 exposed and 36 non-exposed cases studied). An average cumulative exposure was estimated at 10 ppm-years benzene among the exposed. Three p53 mutations were detected by denaturing-gradient gel electrophoresis (DGGE) among the 23 exposed cases (3/23, 13%). Of the non-exposed referent cases, 4 had a mutation (4/30, 13%). All but one of the cases with a p53 mutation had smoked. A ras gene (K-ras or N-ras) mutation was found in 3 (3/66, 4.5%) cases, all of whom were smoker referents. We conclude that p53 and ras mutations are infrequent in renal-cell cancer associated with occupational exposure to gasoline. However, the majority of the mutations (6/7 for p53, and 3/3 for ras genes) were seen in smokers. Int.