S 1 nuclease analysis and sub-family-specific oligonucleotide probes were used to characterize the expression during embryogenesis of the napin storage protein gene family of Brassica napus (oilseed rape). The expression of one sub-class represented by the napin gene gNa peaks and declines earlier t
Molecular analysis of a cruciferin storage protein gene family ofBrassica napus
β Scribed by John P. Breen; Martha L. Crouch
- Book ID
- 104618745
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 765 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0167-4412
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β¦ Synopsis
We have isolated a five-member gene subfamily which encodes cruciferin, a legumin-like 12S storage protein of Brassica napus L., and have analyzed the structure and expression of the family members in developing embryos. Sequence analysis has shown that the coding regions of all five genes are highly similar, with the two most divergent members of the family retaining 89 ~o sequence identity. The analysis of this cruciferin gene family's expression indicates that the developmental pattern of expression of each gene is similar, and the steady-state mRNA levels of each gene are approximately equivalent to each other at all developmental stages.
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