Modulation of α4β1 and α5β1 integrin expression: Heterogeneous effects of Q-switched Ruby, Nd:YAG, and Alexandrite lasers on melanoma cells in vitro
✍ Scribed by van Leeuwen, Robert L.; Dekker, Sybren K.; Byers, H. Randolph; Vermeer, Bert Jan; Grevelink, Joop M.
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 813 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0196-8092
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✦ Synopsis
Background and Objectiue: Integrins of the pl family are cellular adhesion molecules that play an important role in cell attachment and migration by interacting with extracellular matrix molecules. Agents such as hormones, cytokines, and ultraviolet radiation have all been shown to have an integrin modulating potential. The present study indicates that radiation of Qswitched lasers is also able to induce transient changes in integrin expression levels on human melanoma cells in vitro. Study DesignlMaterials and Methods: Radiation from Q-switched Ruby (694 nm), Alexandrite (755 nm), and NdYAG laser (1,064 nm) with fluences comparable to those that are generally used in treating dermatologic lesions were used to irradiate a subconfluent layer of human melanoma cells. After fixed time intervals, the cells were harvested either to analyse the integrin expression by flow cytometry or to investigate changes in cell attachment, spreading, and migration. Results: It was established that all three types of laser were able to cause a significant downregulation of both the a4 and the common pl integrin subunit. The Alexandrite and Ruby lasers also induced a decrease in a5 expression; however, the cells treated with the NdYAG laser showed a marked upregulation of the a5 subunit. The expression of the other p l integrin subunits was shown to be unaltered after laser treatment. Downregulation of the a4 upregulation of the a5 integrin subunit expression resulted in, respectively, decreased and increased attachment and spreading on fibronectin, the extracellular matrix ligand for both the a4pl and a5pl integrins. Marked upregulation of the a5 subunit also resulted in a higher migration rate. Conclusion: Taken together, these results show that nonlethal doses of Q-switched laser radiation are able to induce changes in cellular behavior in Vitro by modulating the integrin expression pattern. o 1996 Wiley-Liss, Inc.