Modulation of the transport of bilirubin and asialoorosomucoid during liver regeneration

✍ Scribed by Dr. Ulrich Gärtner; Richard J. Stockert; Anatol G. Morell; Allan W. Wolkoff

• Book ID: 102850336
• Publisher: John Wiley and Sons
• Year: 1981
• Tongue: English
• Weight: 756 KB
• Volume: 1
• Category: Article
• ISSN: 0270-9139
• DOI: 10.1002/hep.1840010203

✦ Synopsis

The normal rat hepatocyte divides approximately once per year but, following two thirds hepatectomy, rapid cellular replication occurs throughout the remaining liver remnant. Using a multiple indicator dilution technique, single-pass transport of 3H-bilirubin and 1251-asialoorosomucoid was studied in isolated perfused liver from 6 hr to 6 d after two thirds hepatectomy or sham surgery. Influx (kl), efflux (kZ), and sequestration (k3) rates were quantitated by computer analysis. kl for 3H-bilirubin fell by over 50% within 6 hr after two thirds hepatectomy and returned to normal 4 d later. kz progressively decreased with a nadir at 2 d, and returned to normal by 4 d. k3 was transiently depressed, and became normal within 2 d. Although hepatic uptake of asialoglycoproteins has been thought to be irreversible, the experimental data required kz and k3 parameters for best fit. Similar to results for 3H-bilirubin, the kl of 1251-asialoorosomucoid was 20% of normal at 1 d after two thirds hepatectomy, and returned to normal by 6 d. Unlike results for 3H-bilirubin, there was a prolonged 50% reduction of kZ and k3 with return to normal by 6 d. The transport changes during regeneration are independent of reduced liver mass or changes in hepatic spaces of distribution. The fact that influx of both compounds reaches a nadir at the time of greatest cellular proliferation with subsequent return to normal suggests a "maturation" of liver cell function for restoration of these specific hepatocyte functions. Modulation of the hepatocyte receptor for desialylated glycoproteins may also be required for cellular recognition as a prerequisite for proliferative responses.