Modulation of the SOS response by truncated RecA proteins
โ Scribed by Larminat, Florence ;Defais, Martine
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 704 KB
- Volume
- 216
- Category
- Article
- ISSN
- 0026-8925
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The products of the lexA and recA genes play central roles in the regulation of the Escherichia coli SOS response. We have measured the rate of mRNA synthesis from each gene at intervals following various inducing treatments in order to obtain a more precise timing of the induction process. Further,
Cellular activities normally inducible by DNA damage (SOS functions) are expressed, without DNA damage, in recA441 (formerly tif-1) mutants of Escherichia coli at 42 degrees C but not at 30 degrees C. We describe a strain (SC30) that expresses SOS functions (including mutator activity, prophage indu