Modulation of the G0 to S phase transit time by insulin: Potential involvement of protein phosphorylation
✍ Scribed by Rolf F. Kletzien; Philip Day
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 573 KB
- Volume
- 105
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Abstract
BHK fibroblasts can be growth arrested by incubation in low serum (0.1%) medium. Growth is initiated by incubating cells in high serum (10%) medium. We have found that if the quiscent cells in low serum medium are incubated with insulin, the G~0~ to S transit time is decreased by two to six hours when serum (10%) is added back to the culture. The effect of insulin treatment of quiescent cells on the cellular phosphoprotein profile was examined. It was found that insulin stimulated the phosphorylation of a 96,000 dalton cytosol protein. This protein is also intensely phosphorylated in proliferating cells and may be one of the critical intracellular events to occur when a cell initiates growth.