Modulation of the dihydropyridine-sensitive calcium channels inDrosophila by a phospholipase C-mediated pathway

✍ Scribed by Gu, Gang-Guo ;Singh, Satpal

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 269 KB
• Volume: 33
• Category: Article
• ISSN: 0022-3034
• DOI: 10.1002/(sici)1097-4695(199709)33:3<265::aid-neu5>3.0.co;2-#

✦ Synopsis

Disruption of phospholipase C-b PKC, reduced the current in the normal fibers without (PLC) by the norpA mutations of Drosophila renders affecting the mutant current. 300 mM sn-1,2-dioctaflies blind by affecting the light-evoked photoreceptor noyl-glycerol (DOG), an analog of diacylglycerol potential. We report here that the norpA-coded PLC (DAG), increased the current in the mutant fibers. modulates the 1,4-dihydropyridine (DHP)-sensitive These experiments suggest that the DHP-sensitive Ca 2/ channels in larval muscles. The DHP-sensitive Ca 2/ channels in Drosophila may be modulated by the current was reduced in the norpA mutants. Applica-PLC-DAG-PKC pathway, and that the same PLC tion of 1 mM phorbol 12-myristate 13-acetate (TPA) isozyme which is involved in phototransduction in the and 1 mM phorbol 12,13-didecanoate (PDD), activaadult flies may also modulate muscle Ca 2/ channels tors of protein kinase C (PKC), rescued the current in the larval stage of development. ᭧ 1997 John Wiley & in the mutant fibers without significantly affecting