Modulation of immunological properties of allogeneic mesenchymal stem cells by collagen scaffolds in cartilage tissue engineering

✍ Scribed by Tun Yuan; Kuifeng Li; Likun Guo; Hongsong Fan; Xingdong Zhang

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 926 KB
• Volume: 98A
• Category: Article
• ISSN: 1549-3296
• DOI: 10.1002/jbm.a.33121

✦ Synopsis

Abstract

Influence of the structures of some collagen scaffolds on immunological properties of the seeded allogeneic mesenchymal stem cells (MSCs) was studied in this article. Hydrogels, sponge, and membrane were prepared from type‐I collagen. These scaffolds were seeded with neonatal rabbit MSCs and cultured for different periods. Changes of the immunological properties associated with different scaffolds were analyzed and compared. It was found that the expression of major histocompatibility complex (MHC) class I and II molecules on MSCs increased gradually in all scaffolds, but the least increment was recorded in hydrogels. Mixed lymphocyte reactions (MLR) showed that the MSC‐hydrogel constructs invoked considerably low allogeneic lymphocytes proliferation. Even in presence of interferon‐γ (IFN‐γ), the hydrogels with higher concentration gave comparatively lower increment of MHC‐II expression and allogeneic lymphocytes proliferation. These results suggest that different scaffold structures may provide different microenvironments and extents of isolation from the host immune system for the seed cells, thereby affecting their immunological properties. Therefore, scaffold structures may modulate the immunological properties of tissue‐engineered cartilage with allogeneic cells. Hydrogels, especially which were prepared from higher collagen concentrations, were found to be a promising scaffold structure, from the perspective of avoiding severe immune rejection. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2011.