Modulation of gene expression in the preimplantation mouse embryo by TGF-α and TGF-β

✍ Scribed by Gbolagade O. Babalola; Richard M. Schultz

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 765 KB
• Volume: 41
• Category: Article
• ISSN: 1040-452X
• DOI: 10.1002/mrd.1080410203

✦ Synopsis

Abstract

The effect of growth factors on regulating gene expression in the preimplantation mouse embryo was examined, since results of previous experiments revealed a stimulatory effect of exogenously‐added growth factors on preimplantation development in vitro. Treatment of early cavitating blastocysts with either 250 pM TGF‐α or TGF‐β results in changes in the pattern of total protein synthesis as assessed by high‐resolution two‐dimensional gel electrophoresis. In some cases, the synthesis of a particular polypeptide is either up‐ or downregulated by each growth factor, whereas in other instances the synthesis of a polypeptide is modulated by one but not the other growth factor. Use of the mRNA differential display method permitted the identification of genes whose expression is either up‐ or downregulated by these growth factors. Treatment of mouse blastocysts with either TGF‐α or TGF‐β results in the increased expression of the b subunit of the F~0~ ATPase. TGF‐β also stimulates the expression of the DNA polymerase α. TGF‐α treatment results in the increase in expression of a gene homologous to the human HEPG2 cDNA, as well as in a decrease in expression of fibronectin. © 1995 Wiley‐Liss, Inc.