Modulation of gene expression in Escherichia coli infected with single-stranded bacteriophage ϕX174

✍ Scribed by Ghosh, Amit ;Pal, S. K. ;Poddar, R. K.

• Publisher: Springer
• Year: 1985
• Tongue: English
• Weight: 518 KB
• Volume: 198
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00383011

✦ Synopsis

Synthesis of tryptophanase, D-serine deaminase and alkaline phosphatase in Escherichia coli C was repressed as the result of infection with the single-stranded DNA bacteriophage d~X174. However, the degree of repression differed, the more catabolite-sensitive the operon was, the more severe was the repression. For the catabolite-sensitive enzymes it was found that cyclic adenosine 3'5' monophosphate (cyclic AMP or cAMP) was unable to release or reduce the phage-induced inhibition. Experiments with amber mutants of qbX174 revealed that A*, product of cistron A, was responsible for the inhibition. The cistron A product probably acted at the level of transcription. The possible role of A* in the observed modulation of gene expression is discussed.