Modulation of extracellular matrix proteins in rat liver during development

✍ Scribed by Dr. Shimon Reif; Victor P. Terranova; Mamdouh El-Bendary; Emanuel Lebenthal; James K. Petell

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 823 KB
• Volume: 12
• Category: Article
• ISSN: 0270-9139
• DOI: 10.1002/hep.1840120312

✦ Synopsis

The expreaeion and localization of extracellular matrix proteins in rat liver was investigated as a function of liver development. Levels of extracellular matrix proteins were m e d by dot-blot or immunoblot protocols uaing monogPBcWc antibodies prepared against collagen typee I, III and Iv; laminin; fibronectb, and fibronectin receptor. Proline and hydmxyproline levela from extracted liver collagen were quantitated by Pico Tag analyaie. It WM obeerved that the oontent of type IV collagen and fibronectin in the rat liver incre~aeed two to four timw during the perinatal period. In contraat, levels of laminin and collagen typea I and III decreaeed up to twofold in developing rat livers. The content of fibronectin receptor during ontogeny was decreased four timea in an inverse relationship to fibronectin m o l d e a . Fibronectin receptor and extracellular matrix p r o t e h dieplayed no difference in apparent m o l d a r weight as ju@id by eodium dodecyl date-polyacrylamide gel eletctrophoreeie immunoblota. Indirect immunofluoreacence etaining of frozen thin liver sectiona revealed that the pattern of localization of extracellular matrix proteins in the nonvascular MOM of fetal liver was punctate rather than restricted to a epeciflc region such aa the perieinusoidal area of adult livers. Similarly, fibronectin receptor was al.0 present, mainly in the ainusoidal area of adult livens, whereas fetal sectiona were avrUeely stained. Our findings that the differential modulation of exh9cellular matrix proteins and their locallzation in the d d o p i n g rat livers undergo a dramatic alteration in the composition and structural organization of matrix material, which may act to modulate prolifemation and to promote the differentiation of liver ceb during development. (HEPATOLOGY 1990, 12:619-626.)