Modulation of cytokine expression in mouse dendritic cell clones

✍ Scribed by Francesca Granucci; Giampiero Girolomoni; Manfred B. Lutz; Maria Foti; Giulia Marconi; Paola Gnocchi; Luisa Nolli; Paola Ricciardi-Castagnoli

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 567 KB
• Volume: 24
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830241039

✦ Synopsis

Modulation of cytokine expression in mouse dendritic cell clones*

Dendritic cells (DC) play an essential role in the induction of primary immune responses; howeveqvery little information is available on cytokine production by DC. Here we determined the cytokine gene expression profile of two immortalized DC clones, CBI and D2SC/1, both generated from mouse spleen but differing in their activation requirements. Among the cytokines tested, only transforming growth factor-P1 was transcribed constitutively, but its production was detected only in D2SC/1 cells after treatment with granulocytelmacrophage colony-stimulating factor (GM-CSF). GM-CSF also promoted transcription and synthesis of interleukin (IL)-lB in CB1 cells that need pretreatment with GM-CSF to present major histocompatibility complex class II-restricted antigens efficiently in vitro. Lipopolysaccharide (LPS) up-regulated gene expression and induced release of tumor necrosis factor-cx in both DC clones. In addition, LPS induced transcription of IL-la and both gene expression and synthesis of IL-lP in D2SC/l cells. Interferon-y was ineffective in inducing cytokine gene expression, although it augmented the antigen-presentation capacity of DC. IL-4, IL-10 and IL-12 mRNA were not induced by any of the tested stimuli. The results suggest that DC have a limited cytokine gene expression pattern compared to macrophages and are heterogenous in some functional properties. * This work was partly supported by the Italian Association for Cancer Research (AIRC), by Biotop and by Consiglio Nazionale delle Ricerchc (ACRO project).