Modulation by dietary vitamin E and selenium of clotting whole blood thromboxane A2 and aortic prostacyclin synthesis in rats
✍ Scribed by Mohsen Meydani; Simin Nikbin Meydani; Jeffrey B. Blumberg
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 472 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0955-2863
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✦ Synopsis
The effect of dietary vitamin E and selenium (Se) on aortic prostacyclin (PGI2) and clotting whole blood thromboxane (TX) A2 synthesis in 1-month-old Fischer 344 rats was investigated. Rats were fed basal semi-purified diets deficient in vitamin E and Se or basal diet supplemented with either 200 IU vitamin E and~or 0.2 ppm Se per kg diet for 2 months. Ex vivo production of TXB2 and 6-keto-PGF~,, the corresponding stable metabolites of TXA2 and PGlz, were measured in whole blood and aortic rings, respectively. Animals fed vitamin E-deficient diet had significantly (P < 0.05) lower plasma levels of a-tocopherol than those fed vitamin E-supplemented diet. Plasma Se was undetected and activity of plasma glutathione peroxidase was diminished in rats fed Se-deficient diets. Vitamin E and Se treatments affected TXA2 and PGI2 production differently. Aortic PGI2 synthesis was affected by both dietary vitamin E and Se, whereas whole blood TXA2 synthesis was only affected by dietary vitamin E. These results suggest that vitamin E and Se have specific and different effects on TXA2 and PGI2 synthesis, and that their combined supplementation may have a favorable effect on PGI2:TXA2 ratio.