Modulating the binding properties of an anti-17β-estradiol antibody by systematic mutation combinations

Functional characterization of an anti-e

Functional characterization of an anti-estradiol antibody by site-directed mutagenesis and molecular modelling: modulation of binding properties and prominent role of the VL domain in estradiol recognition

✍ Stéphane Coulon; Jean-Luc Pellequer; Thierry Blachère; Martine Chartier; Elisabe 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 288 KB

The high-affinity monoclonal anti-estradiol antibody 9D3 presents a specificity defect towards estradiol-3sulphate and 3-glucuronide conjugates incompatible with use in direct immunoassays. The corresponding single-chain variable fragment (scFv), cloned and produced in E. coli, exhibited a 10-fold l