Modulating cell adhesion and spreading by control of FnIII7–10 orientation on charged self-assembled monolayers (SAMs) of alkanethiolates
✍ Scribed by Hua Wang; Yi He; Buddy D. Ratner; Shaoyi Jiang
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 286 KB
- Volume
- 77A
- Category
- Article
- ISSN
- 1549-3296
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✦ Synopsis
Abstract
In this work, we demonstrate that surface charge can be used to modulate cell adhesion/spreading through the control of the orientation of adsorbed FnIII~7–10~, which is a cell‐adhesive protein containing RGD residues. Carboxylic acid (COOH) and amine (NH~2~)‐terminated self‐assembled monolayers (SAMs) of alkanethiolates were used as model negatively and positively charged surfaces, respectively. The adsorbed amount of FnIII~7–10~ is controlled to be equivalent on both SAMs as confirmed by the adsorption isotherms determined using I^125^‐radiolabeled FnIII~7–10.~ The binding of a monoclonal antibody specific for the cell‐binding domain of FnIII~7–10~ was measured by surface plasmon resonance (SPR) to evaluate FnIII~7–10~ orientations on different SAMs. Results indicate that adsorbed FnIII~7–10~ on NH~2~‐SAM has an orientation with more cell‐binding domains accessible than on COOH‐SAM, confirming our predictions from Monte Carlo simulations. Both phase contrast images and Vybrant® MTT cell proliferation assays show that the adhesion/spreading of bovine aortic endothelial cells (BAECs) on the NH~2~‐SAM is significantly better than that on the COOH‐SAM coated with an equivalent amount of FnIII~7–10~. These results indicate that surface charge can be used to specifically orient cell adhesive proteins such as FnIII~7–10~, thus providing a promising strategy to increase the activity of materials incorporating biological moieties. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006