We have critically evaluated various modifications of the alkaline elution methodology that were required to adapt the method for measuring DNA damage in cells from animal tissues treated in vivo. These modifications involved the use of a fluorometric assay for the eluted DNA using the dye Hoechst 3
โฆ LIBER โฆ
Modifying the function of DNA repair nanomachines for therapeutic benefit
โ Scribed by William S. Dynan; Yoshihiko Takeda; Shuyi Li
- Publisher
- Elsevier Science
- Year
- 2006
- Tongue
- English
- Weight
- 296 KB
- Volume
- 2
- Category
- Article
- ISSN
- 1549-9634
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## Abstract Germline mutations in DNA mismatch repair genes underlie one of the most common hereditary cancer predisposition syndromes known in humans, hereditary nonpolyposis colorectal cancer (HNPCC). Defects of the DNA mismatch repair system are also prevalent in sporadic colorectal cancers. The