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Modified methods for preparation of cryostat sections of skeletal muscle

✍ Scribed by Dr. Ju-Sung Wu; Gwendolyn R. Hogan; Jerry D. Morris


Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
462 KB
Volume
8
Category
Article
ISSN
0148-639X

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A new technique is presented for the external stabilization of cryostat sections by spraying the specimen surfaces with an aqueous solution of poly(vinyl alcohol) before each sectioning stroke. The spray freezes upon the surface and forms a tough coating which facilitates subsequent sectioning and h