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Modified lipase having high stability and various enzymic activities in benzene, and its re-use by recovering from benzene solution

✍ Scribed by T. Yoshimoto; K. Takahashi; H. Nishimura; A. Ajima; Y. Tamaura; Y. Inada


Publisher
Springer Netherlands
Year
1984
Tongue
English
Weight
209 KB
Volume
6
Category
Article
ISSN
0141-5492

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✦ Synopsis


Lipoprotein

lipase modified with polyethylene glycol dissolved in benzene, and catalyzed various reactions of ester synthesis, ester exchange and aminolysis.

This modified enzyme had a high stability; 50% of the initial enzymic activity were retained after about 3 months-storage in benzene at room temperature.

We can repeatedly re-use the enzyme by recovering from benzene solution; n-hexane(or petroleum ether).

the enzyme precipitates upon addition of