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Modified assay procedure for the estimation of serum glucose using microwell reader

โœ Scribed by M. Srikanth; G. Venkateswara Rao; K. R. S. Sambasiva Rao


Publisher
Association of Clinical Biochemistry of India
Year
2004
Tongue
English
Weight
148 KB
Volume
19
Category
Article
ISSN
0970-1915

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We developed an assay for serum magnesium (Mg) by coupling phosphoglucomutase (EC 2.7.5.1) with glucose-6-phosphate dehydrogenase (EC 1.1.1.49). The kinetic generation of NADPH by the action of the above two enzymes upon glucose-l-phosphate (Gl-P) and glucoso-l,6-diphosphate (G-1,6-P) was proportion