A new four-color flow cytometric assay t
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H.J. Hasper; R.M. Weghorst; D.J. Richel; J.H. Meerwaldt; F.M.F.G. Olthuis; C.E.I
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Article
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2000
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John Wiley and Sons
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English
β 214 KB
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Background: Human peripheral blood lymphocytes kept in culture after isolation die by an apoptotic process. Detection of apoptosis with labeled Annexin V to demonstrate loss of plasma membrane asymmetry is sensitive, specific, and easy using flow cytometry. This is true in lymphoblastic cell lines w