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Modifications in flow cytometric estimation of t cell subsets and b cells in peripheral blood to reduce the cost of investigation

✍ Scribed by Sanju Jalla; Sunil Sazawal; Saikat Deb; Robert E. Black; Maharaj K. Bhan


Publisher
Association of Clinical Biochemistry of India
Year
2002
Tongue
English
Weight
435 KB
Volume
17
Category
Article
ISSN
0970-1915

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Background: Human peripheral blood lymphocytes kept in culture after isolation die by an apoptotic process. Detection of apoptosis with labeled Annexin V to demonstrate loss of plasma membrane asymmetry is sensitive, specific, and easy using flow cytometry. This is true in lymphoblastic cell lines w