Modification of USP vitamin D assay to remove interference of vitamin E

✍ Scribed by Modest Osadca; Elmer De Ritter

• Book ID: 102399311
• Publisher: John Wiley and Sons
• Year: 1968
• Tongue: English
• Weight: 366 KB
• Volume: 57
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600570213

✦ Synopsis

When tocopherol is incorporated in multivitamin products at the 10-30 mg. levels, corresponding to the daily adult requirement, serious interference is encountered in determining vitamin D by the USP method. A modification of the USP assay method is described in which the tocopherol is separated by column chromatography on secondary magnesium phosphate prior to the normal USP chromatographic purification steps. Proper conditioning and performance testing of the secondary magnesium phosphate are essential to assure good separation of tocopherol and quantitative recovery of vitamin D.