Modification of tryptophan 149 of inorganic pyrophosphatase from Escherichia coli
β Scribed by Shun-Ichi Kaneko; Tetsuroh Ichiba; Naoto Hirano; Akira Hachimori
- Book ID
- 103122948
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 606 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0020-711X
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## Abstract Postβtranscriptional modifications of bases within the transfer RNAs (tRNA) anticodon significantly affect the decoding system. In bacteria and eukaryotes, uridines at the wobble position (U34) of some tRNAs are modified to 5βmethyluridine derivatives (xm^5^U). These xm^5^U34βcontaining
Escherichia coli B 10, which has high activity of tryptophan synthetase, was grown in a 50-L batch culture in order to determine in which growth phase the cells have the highest specific tryptophan productivity. Accordingly, whole cells of the stationary phase were used for immobilization in polyacr