Modification of the Waaler-Rose test using formalinized sheep erythrocytes
✍ Scribed by Felix Milcrom; Olav Tonder; Ulana Loza
- Publisher
- John Wiley and Sons
- Year
- 1964
- Tongue
- English
- Weight
- 441 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0004-3591
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✦ Synopsis
A slide modification of the Waaler-Esseva elaborate un lamina modification Rose test was developed using formalin-del test de Waaler-Rose, con le us0 de ized sheep erythrocytes. Good agree-formalinisate erythrocytos ovin. Esseva ment of the results obtained with the notate un bon grado de concordantia con standard procedure and the slide modi-le methodo standard e le modification a fication was observed. lamina porta-objectos. EROLOGICAL TESTS for rheumatoid arthritis have been routinely used S for the last 10 years as valuable diagnostic tools. All these tests are based on the detection of a serum factor called the rheumatoid factor which has serological properties of an antibody against gamma g1obulin.l The first of these tests described by Waaler2 and by Rose et al.,3 employes sheep erythrocytes sensitized by subagglutinating doses of a rabbit antiserum; the sensitized red blood cells are agglutinated by the rheumatoid factor. The Waaler-Rose
test is being replaced more and more by the much simpler latex test in which the rheumatoid factor agglutinates latex particles coated by human gamma g l ~b u l i n . ~ However, the Waaler-Rose test is still a valuable serodiagnostic procedure. It appears that this test is somewhat less sensitive, but more specific for rheumatcid arthritis than the latex test."8 Waller and her collaboratorsR found the Waaler-Rose test positive in 68 per cent of patients suffering from rheumatoid arthritis, whereas the latex test was positive in 86 per cent. In testing 340 apparently healthy blood donors, the same authors found the Waaler-Rose test positive in only one case (0.3 per cent) but the latex test was positive in 10 cases ( 3 per cent). A high prevalence of positive latex tests in older persons was reported by Heimer and Rudd.7 In testing 101 aged people the authors found the latex test positive in 50 persons. Only in one case the positive test was justified by a clinical diagnosis of rheumatoid arthritis. In the same material only two persons were positive by the Waaler-Rose test.
This situation may be explained by the fact that anti-gamma gIobulin fac-